| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Taylor, Kayana R (14)
|
NCYSST |
56.02 |
58.52 |
2.50 |
| 2 |
Edwards, Emma K (13)
|
NCYSST |
58.25 |
59.61 |
1.36 |
| 3 |
Durak, Gwenyth B (14)
|
NCYSST |
59.19 |
1:00.15 |
0.96 |
| 4 |
Hernandez-Pena, Valeria (13)
|
NCYSST |
1:03.03 |
1:00.44 |
-2.59 |
| 5 |
Littauer, Rebekah H (18)
|
NCWNCY |
58.67 |
1:02.65 |
3.98 |
| 6 |
Cook, Catelyn M (14)
|
NCYSST |
1:11.73 |
1:05.92 |
-5.81 |
| 7 |
Bemis, Olivia L (13)
|
NCWNCY |
1:05.40 |
1:06.85 |
1.45 |
| 8 |
Hixson, Kaelyn R (16)
|
NCWNCY |
1:07.32 |
1:06.96 |
-0.36 |
| 9 |
Bolick, Greer J (13)
|
NCYSST |
1:06.03 |
1:06.98 |
0.95 |
| 10 |
Shlien-Dellinger, Phoenix S (13)
|
NCWNCY |
1:06.98 |
1:07.35 |
0.37 |
| 11 |
Farmer, Sydney A (15)
|
NCYSST |
1:09.11 |
1:08.11 |
-1.00 |
| 12 |
Faherty, Taylor R (17)
|
NCYSST |
|
1:12.48 |
- |
| 13 |
Allison, Alexis D (16)
|
NCYSST |
1:18.18 |
1:16.76 |
-1.42 |
| 15 |
Tuttle, Mikayla R (13)
|
NCWNCY |
|
1:20.82 |
- |