| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Setzer, Sean T (12)
|
NCWOW |
33.05 |
33.28 |
0.23 |
| 2 |
Alexander, Evan M (12)
|
NCWAVE |
34.51 |
34.70 |
0.19 |
| 3 |
Christiansen, Nicolas G (12)
|
NCHCAC |
39.11 |
37.39 |
-1.72 |
| 4 |
Durham, William L (12)
|
NCWOW |
38.68 |
37.67 |
-1.01 |
| 5 |
Bennison, James W (12)
|
NCWAVE |
38.21 |
38.01 |
-0.20 |
| 6 |
Hershfeld, William T (12)
|
NCWAVE |
48.17 |
39.33 |
-8.84 |
| 7 |
Cook, Hunter T (12)
|
NCWAVE |
40.88 |
39.86 |
-1.02 |
| 8 |
Hughes, Jackson C (11)
|
NCWOW |
40.54 |
40.06 |
-0.48 |
| 9 |
Langtry, Ryan C (12)
|
NCWOW |
47.10 |
41.59 |
-5.51 |
| 10 |
Post, Cian D (11)
|
NCWOW |
43.75 |
43.69 |
-0.06 |
| 11 |
Fary, Elliott K (11)
|
NCCFAC |
50.79 |
44.95 |
-5.84 |
| 12 |
Eaton, Samuel R (11)
|
NCHCAC |
45.36 |
45.00 |
-0.36 |
| 13 |
Pease, Andrew J (12)
|
NCACCH |
46.43 |
45.61 |
-0.82 |
| 14 |
Ball, Deacon T (11)
|
NCCFAC |
47.11 |
45.72 |
-1.39 |
| 15 |
Donaldson, Miles D (11)
|
NCCAST |
|
46.00 |
- |
| 16 |
Eberhardt, Nathan L (12)
|
NCWOW |
46.00 |
46.25 |
0.25 |
| 17 |
Kashmer, Christian J (11)
|
NCCAST |
48.45 |
46.38 |
-2.07 |
| 18 |
Wang, Maximilian (11)
|
NCWOW |
49.52 |
46.79 |
-2.73 |
| 19 |
Mack, Dawson L (12)
|
NCHCAC |
46.00 |
47.58 |
1.58 |
| 20 |
Tillery, Wade H (11)
|
NCWOW |
51.48 |
48.32 |
-3.16 |
| 21 |
Kanoy, Owen D (12)
|
NCCFAC |
1:09.94 |
50.81 |
-19.13 |
| 22 |
Page, Logan A (11)
|
NCWAVE |
49.88 |
51.46 |
1.58 |
| 23 |
Mejouev, Maxim (11)
|
NCWOW |
50.75 |
51.67 |
0.92 |
| 24 |
Sorensen, Nikolas P (12)
|
NCBAC |
49.56 |
51.93 |
2.37 |
| 25 |
Hunt, Josiah J (11)
|
NCCFAC |
|
53.83 |
- |
| 26 |
Nelson, Gabriel B (12)
|
NCSSS |
|
58.77 |
- |
| 27 |
Pace, Andrew N (11)
|
NCBAC |
1:17.43 |
1:02.63 |
-14.80 |
|
Hurlbert, Cole R (12)
|
NCWAVE |
39.81 |
DQ |
- |
|
Reddy, Dorian K (12)
|
NCBAC |
|
DQ |
- |
|
Louviere, Sage M (12)
|
NCBAC |
|
DQ |
- |
|
Jaynes, Bradley T (11)
|
NCBAC |
1:10.12 |
NS |
- |