| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thompson, William E (12)
|
NCACCH |
28.58 |
28.51 |
-0.07 |
| 2 |
Doherty, Torin N (12)
|
NCWAVE |
29.18 |
28.68 |
-0.50 |
| 3 |
Bennison, James W (12)
|
NCWAVE |
29.55 |
29.83 |
0.28 |
| 4 |
Christiansen, Nicolas G (12)
|
NCHCAC |
32.42 |
30.09 |
-2.33 |
| 5 |
Hurlbert, Cole R (12)
|
NCWAVE |
29.21 |
30.34 |
1.13 |
| 6 |
Cook, Hunter T (12)
|
NCWAVE |
32.07 |
31.06 |
-1.01 |
| 7 |
Durham, William L (12)
|
NCWOW |
32.24 |
31.11 |
-1.13 |
| 8 |
Stuck, Evan J (12)
|
NCWOW |
31.11 |
31.13 |
0.02 |
| 9 |
Setzer, Sean T (12)
|
NCWOW |
31.44 |
31.28 |
-0.16 |
| 10 |
Langtry, Ryan C (12)
|
NCWOW |
32.47 |
31.93 |
-0.54 |
| 11 |
Hughes, Jackson C (11)
|
NCWOW |
32.94 |
32.00 |
-0.94 |
| 12 |
Post, Cian D (11)
|
NCWOW |
36.67 |
34.44 |
-2.23 |
| 13 |
Marsteiner, Matthew F (11)
|
NCWAVE |
34.61 |
34.86 |
0.25 |
| 14 |
Mack, Dawson L (12)
|
NCHCAC |
41.00 |
35.71 |
-5.29 |
| 15 |
Fary, Elliott K (11)
|
NCCFAC |
|
36.84 |
- |
| 15 |
Eberhardt, Nathan L (12)
|
NCWOW |
39.11 |
36.84 |
-2.27 |
| 17 |
Witmer, William C (11)
|
NCCFAC |
48.41 |
37.53 |
-10.88 |
| 18 |
Kanoy, Owen D (12)
|
NCCFAC |
|
38.52 |
- |
| 19 |
Williams, Hatcher M (12)
|
NCACCH |
36.90 |
38.68 |
1.78 |
| 20 |
Whisnant, Mitchell W (11)
|
NCWOW |
46.78 |
39.48 |
-7.30 |
| 21 |
Tillery, Wade H (11)
|
NCWOW |
40.64 |
40.05 |
-0.59 |
| 22 |
Hunt, Josiah J (11)
|
NCCFAC |
|
42.19 |
- |
| 23 |
Brown, Noah D (11)
|
NCWOW |
48.97 |
45.23 |
-3.74 |
| 24 |
Pease, Andrew J (12)
|
NCACCH |
53.69 |
45.70 |
-7.99 |
| 25 |
Mejouev, Maxim (11)
|
NCWOW |
47.69 |
46.87 |
-0.82 |
| 26 |
Wang, Maximilian (11)
|
NCWOW |
50.32 |
50.06 |
-0.26 |
| 27 |
Pace, Andrew N (11)
|
NCBAC |
58.57 |
53.05 |
-5.52 |
|
Anderson, Crawford T (10)
|
NCCFAC |
|
DQ |
- |