| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mitchell, Lillian B (14)
|
NCCVAC |
2:17.33 |
2:19.03 |
1.70 |
| 2 |
Ku, Katherine E (14)
|
NCTEAM |
2:18.53 |
2:20.05 |
1.52 |
| 3 |
Hefner, Caitlin L (13)
|
NCYSST |
2:18.42 |
2:21.92 |
3.50 |
| 4 |
Wenzel, Trinity A (12)
|
NCSMAC |
2:28.31 |
2:24.65 |
-3.66 |
| 5 |
Walsh, Catherine G (12)
|
NCMAC |
2:26.96 |
2:29.70 |
2.74 |
| 6 |
Hollifield, Meredith V (13)
|
NCRCST |
2:32.45 |
2:35.02 |
2.57 |
| 7 |
Moore, Sarah T (14)
|
NCWNCY |
2:44.61 |
2:41.08 |
-3.53 |
| 8 |
Zarins, Chloe G (14)
|
NCTEAM |
2:37.80 |
2:41.72 |
3.92 |
| 9 |
Strauss, Sydney G (13)
|
NCMAC |
2:52.17 |
2:42.84 |
-9.33 |
| 10 |
McCormack, Olivia A (13)
|
NCWNCY |
2:47.01 |
2:44.80 |
-2.21 |
| 11 |
Kelty, Sarah C (14)
|
NCYSST |
2:41.39 |
2:44.91 |
3.52 |
| 12 |
Deshpande, Annika U (13)
|
NCMAC |
|
2:53.14 |
- |
| 13 |
Oberlin-Pope, Allison G (11)
|
NCTEAM |
|
2:56.34 |
- |
| 14 |
Wheeler, Meghan E (14)
|
NCTEAM |
|
3:03.96 |
- |
| 15 |
Utter, Katelynn P (13)
|
NCYSST |
|
3:18.92 |
- |
| 16 |
Chira, Emanuela N (13)
|
NCYSST |
|
3:23.16 |
- |
|
Cook, Catelyn M (13)
|
NCYSST |
|
DQ |
- |