| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Willis, Aaron N (9)
|
SCYCSC |
39.08 |
36.44 |
-2.64 |
| 2 |
Wolf, Carson J (10)
|
SCRAYS |
38.59 |
37.24 |
-1.35 |
| 3 |
Armbrust, Fletcher J (10)
|
SCRAYS |
41.59 |
37.26 |
-4.33 |
| 6 |
Garrett, Logan F (10)
|
NCYBAC |
38.60 |
38.63 |
0.03 |
| 7 |
KuKucka, Gabriel J (10)
|
SCYCSC |
42.45 |
39.20 |
-3.25 |
| 8 |
Fields, Charles W (10)
|
NCYSST |
41.79 |
41.97 |
0.18 |
| 9 |
Jones, Noah C (9)
|
NCYBAC |
47.32 |
43.23 |
-4.09 |
| 10 |
Petruzzi, Cooper J (8)
|
NCTYDE |
51.61 |
43.63 |
-7.98 |
| 11 |
Hawkins, William H (9)
|
SCYCSC |
47.70 |
44.02 |
-3.68 |
| 12 |
Aberle, Luke T (10)
|
NCYSST |
52.16 |
44.04 |
-8.12 |
| 13 |
McCrae, John R (9)
|
SCRAYS |
41.91 |
44.05 |
2.14 |
| 14 |
Murphy, Dylan H (8)
|
SCYCSC |
50.48 |
45.24 |
-5.24 |
| 16 |
Hessong, Carter A (9)
|
NCYSST |
46.35 |
47.49 |
1.14 |
| 17 |
Kafitz, Brandon J (7)
|
NCRACY |
49.40 |
50.12 |
0.72 |
| 18 |
Kelty, Maximillian C (8)
|
NCYSST |
53.88 |
51.05 |
-2.83 |
| 19 |
Jarrett, Alexander J (9)
|
NCWNCY |
49.95 |
55.81 |
5.86 |
| 20 |
McClam, Marion C (6)
|
NCYSST |
1:04.09 |
58.35 |
-5.74 |
| 21 |
Hawkins III, Rodney D (8)
|
NCRACY |
1:03.18 |
1:00.79 |
-2.39 |
|
Martin, Brandon M (10)
|
SCRAYS |
43.45 |
DQ |
- |