| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Otto, Luke T (21)
|
NISTAR |
2:23.19 |
2:38.33 |
15.14 |
| 2 |
De Livera, Avishka J (15)
|
NIUN-D |
|
2:50.71 |
- |
| 3 |
Duszynski, Andrew M (23)
|
NISTAR |
2:49.56 |
2:50.72 |
1.16 |
| 4 |
Clough, Michael J (17)
|
NISTAR |
2:55.02 |
2:53.35 |
-1.67 |
| 5 |
Tallents, Mark A (15)
|
NIUN-M |
|
2:58.25 |
- |
| 6 |
Yost, Anthony J (17)
|
NIMETS |
2:44.10 |
2:58.99 |
14.89 |
| 7 |
Xiao, Haoheng (15)
|
NIUN-D |
2:57.00 |
3:01.33 |
4.33 |
| 8 |
Staples, Graeme A (15)
|
NISTAR |
2:58.21 |
3:03.92 |
5.71 |
| 9 |
Matteson, Carter J (16)
|
NISTAR |
3:01.10 |
3:04.29 |
3.19 |
| 10 |
Bronson, Dylan T (15)
|
NISDSC |
3:09.11 |
3:06.84 |
-2.27 |
| 11 |
Vannelli, Mitchell T (15)
|
NICLSC |
3:07.37 |
3:07.77 |
0.40 |
| 12 |
Clouser, Kameron D (17)
|
NIBCSR |
2:47.75 |
3:08.53 |
20.78 |
| 13 |
Blake, Joshua C (17)
|
NISTAR |
3:00.56 |
3:15.18 |
14.62 |
| 14 |
Baxter, Samuel J (15)
|
NITTSC |
|
3:18.51 |
- |
| 15 |
Blake, Joseph C (15)
|
NISTAR |
3:04.27 |
3:19.18 |
14.91 |
| 16 |
Murray, Maxwell P (15)
|
NISDSC |
3:08.02 |
3:20.38 |
12.36 |
| 17 |
Morgan, Phillip A (15)
|
NIMERC |
3:14.02 |
3:21.31 |
7.29 |
| 18 |
McCall, Akhil J (16)
|
NIBCSR |
3:29.79 |
3:29.43 |
-0.36 |
| 19 |
Descovich, Nicholas P (16)
|
NITWST |
3:41.22 |
3:30.29 |
-10.93 |
| 20 |
Nienhaus, Jack (15)
|
NISHAQ |
|
3:32.02 |
- |
| 21 |
LeFever, Nathan M (16)
|
NIRAYS |
3:42.35 |
4:11.35 |
29.00 |
|
Wiegand, Jakob W (15)
|
NITTSC |
3:16.93 |
DQ |
- |
|
Deren, Drew E (18)
|
NISTAR |
3:10.48 |
NS |
- |
|
Boglioli, James D (15)
|
NICLSC |
4:32.69 |
NS |
- |
|
Strittmatter, Maximilian K (17)
|
NISTAR |
3:01.88 |
NS |
- |