| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dunn, Matthew E (10)
|
NIHHSC |
33.24 |
32.38 |
-0.86 |
| 2 |
Amer, Sirajedeen F (10)
|
NIVELO |
33.31 |
33.01 |
-0.30 |
| 3 |
Slavinski, William J (10)
|
NIFKVL |
33.36 |
33.15 |
-0.21 |
| 4 |
LaJeunesse, Peter IV F (10)
|
NIVELO |
34.57 |
34.44 |
-0.13 |
| 5 |
Cerasani, Asa J (9)
|
NISWAT |
34.97 |
34.62 |
-0.35 |
| 5 |
Hediger, Kobe K (9)
|
NIAAAC |
35.03 |
34.62 |
-0.41 |
| 7 |
Spence, Matthew M (10)
|
NISHAQ |
35.24 |
34.91 |
-0.33 |
| 8 |
Conner, Peyton J (8)
|
NIGRAT |
36.48 |
34.94 |
-1.54 |
| 9 |
Garcia, Brady E (9)
|
NITTSC |
34.76 |
34.99 |
0.23 |
| 10 |
Anderson, Max (8)
|
NIVELO |
35.83 |
35.71 |
-0.12 |
| 11 |
Farrington, Tyrnan T (10)
|
NIFKVL |
35.28 |
35.94 |
0.66 |
| 12 |
Matteson, Cyrus L (9)
|
NISTAR |
34.57 |
36.10 |
1.53 |
| 13 |
Coen, Liam C (10)
|
NISTAR |
36.29 |
37.42 |
1.13 |
| 14 |
Yang, Gary (9)
|
NIVELO |
37.71 |
37.96 |
0.25 |
| 15 |
Coen, Jack D (10)
|
NISTAR |
37.65 |
40.91 |
3.26 |
| 16 |
Porterfield, Jack D (7)
|
NITTSC |
43.94 |
41.01 |
-2.93 |
| 17 |
Wojciechowski, Trent (9)
|
NIHAMB |
41.56 |
41.15 |
-0.41 |
| 18 |
Carducci, Austin J (10)
|
NITWST |
42.70 |
41.91 |
-0.79 |
|
Coen, Mitchell R (8)
|
NISTAR |
42.70 |
NS |
- |
|
Eisenmann, Joshua T (9)
|
NISTAR |
37.63 |
NS |
- |
|
O'Brien, Kevin J (9)
|
NICLSC |
35.26 |
NS |
- |