| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peyser, Peter M (9)
|
NIBAAC |
40.28 |
39.30 |
-0.98 |
| 2 |
Spence, Matthew M (10)
|
NISHAQ |
40.66 |
39.59 |
-1.07 |
| 3 |
LaJeunesse, Peter IV F (10)
|
NIVELO |
42.77 |
39.76 |
-3.01 |
| 4 |
Yuan, Albert (10)
|
NITTSC |
49.19 |
40.11 |
-9.08 |
| 5 |
Eisenmann, Joshua T (9)
|
NISTAR |
43.88 |
41.44 |
-2.44 |
| 6 |
Slavinski, William J (10)
|
NIFKVL |
43.91 |
41.70 |
-2.21 |
| 7 |
Conner, Peyton J (8)
|
NIGRAT |
44.81 |
42.08 |
-2.73 |
| 8 |
Anderson, Max (8)
|
NIVELO |
47.06 |
43.00 |
-4.06 |
| 9 |
Coen, Liam C (10)
|
NISTAR |
43.78 |
44.20 |
0.42 |
| 10 |
Hediger, Kobe K (9)
|
NIAAAC |
44.64 |
45.28 |
0.64 |
| 11 |
Matteson, Cyrus L (9)
|
NISTAR |
43.57 |
45.42 |
1.85 |
| 12 |
Cvijanovic, Luka (9)
|
NIHAMB |
45.19 |
46.13 |
0.94 |
| 13 |
Porterfield, Jack D (7)
|
NITTSC |
46.29 |
48.28 |
1.99 |
|
Fan, Justin F (10)
|
NICLSC |
44.00 |
NS |
- |
|
Coen, Mitchell R (8)
|
NISTAR |
47.11 |
NS |
- |