| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boyer, Chloe G (8)
|
NIISWM |
34.65 |
35.00 |
0.35 |
| 2 |
Beers, Colsten S (9)
|
NICVSC |
38.21 |
36.31 |
-1.90 |
| 3 |
Fitzgerald, Nora M (9)
|
NICSCI |
36.74 |
36.99 |
0.25 |
| 4 |
Ogata, Olivia Y (9)
|
NISYCH |
38.86 |
37.72 |
-1.14 |
| 5 |
Sammon, Caitlyn I (9)
|
NICSCI |
36.67 |
37.77 |
1.10 |
| 6 |
Bush, Mary (9)
|
NICAGO |
37.28 |
38.51 |
1.23 |
| 7 |
Cheresnowsky, Olivia K (8)
|
NIWAAC |
37.66 |
38.59 |
0.93 |
| 8 |
Murphy, Gabriela (8)
|
NIHHTA |
39.83 |
39.50 |
-0.33 |
| 9 |
El Ghissassi, Sidra S (7)
|
NIVEST |
40.13 |
40.25 |
0.12 |
| 10 |
Phoha, Rekha W (9)
|
NISYCH |
39.89 |
42.02 |
2.13 |
|
Kuznia, Arianna M (9)
|
NIJETS |
36.81 |
DQ |
- |
|
Seidberg, Ashley M (9)
|
NISYCH |
35.57 |
DQ |
- |