| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gorton, Claire P (11)
|
NIWEBS |
33.79 |
33.04 |
-0.75 |
| 2 |
Kazmark, Olivia N (11)
|
NIVICT |
36.55 |
33.92 |
-2.63 |
| 3 |
Newsome, Amber G (11)
|
NIPMSC |
36.67 |
35.01 |
-1.66 |
| 4 |
Williams, Sarah E (11)
|
NIHAST |
38.57 |
35.83 |
-2.74 |
| 5 |
Huerta-Andrade, Isabella (11)
|
NIFAST |
38.30 |
36.04 |
-2.26 |
| 6 |
Zerger, Belah (11)
|
NIGRSC |
35.27 |
36.17 |
0.90 |
| 7 |
Childs, Maggie E (11)
|
NIWEBS |
37.19 |
37.36 |
0.17 |
| 8 |
Buzzell, Ella L (11)
|
NIGRSC |
38.44 |
38.15 |
-0.29 |
| 9 |
Santmyer, Grace E (11)
|
NISDSC |
40.50 |
38.37 |
-2.13 |
| 10 |
Bugbee, Kyla C (11)
|
NIPMSC |
41.37 |
38.65 |
-2.72 |
| 11 |
Taberski, Maura J (11)
|
NIIRON |
1:16.44 |
39.40 |
-37.04 |
| 12 |
Rozalski, Jasmine L (11)
|
NIMERC |
38.14 |
40.63 |
2.49 |
| 13 |
Stonehouse, Sierra L (11)
|
NIGRSC |
45.38 |
45.70 |
0.32 |
| 14 |
Cabral, Aurora (11)
|
NIRRST |
55.74 |
46.95 |
-8.79 |
| 15 |
Kenny, Elisabeth A (11)
|
NIUN |
47.66 |
47.30 |
-0.36 |