| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huang, Cailey (9)
|
NIPACK |
45.84 |
39.27 |
-6.57 |
| 2 |
Anthony, Jillian M (9)
|
NIPMSC |
42.14 |
40.60 |
-1.54 |
| 3 |
Martin, Meghan H (9)
|
NIWEBS |
42.75 |
40.69 |
-2.06 |
| 4 |
Ritter, Madeleine (7)
|
NIPACK |
42.25 |
41.03 |
-1.22 |
| 5 |
Gilbert, Natalie J (8)
|
NIUN |
41.98 |
41.38 |
-0.60 |
| 6 |
Crowley, Lila M (8)
|
NIGRSC |
42.42 |
43.45 |
1.03 |
| 7 |
Huerta-Andrade, Amanda (9)
|
NIFAST |
49.39 |
43.65 |
-5.74 |
| 8 |
Ashby, Emma M (8)
|
NISDSC |
47.82 |
43.82 |
-4.00 |
| 9 |
van Wichen, Jessica D (9)
|
NIWEBS |
45.70 |
44.06 |
-1.64 |
| 10 |
Phillips, Hayley Q (9)
|
NIIRON |
46.12 |
44.27 |
-1.85 |
| 11 |
Manciocchi, Kayden M (8)
|
NIGRSC |
|
44.40 |
- |
| 12 |
Mantell, Norah A (7)
|
NIIRON |
1:07.28 |
44.66 |
-22.62 |
| 13 |
Buzzell, Emily H (8)
|
NIGRSC |
|
46.60 |
- |
| 14 |
LaDelfa, Sophia L (9)
|
NIWEBS |
49.81 |
47.80 |
-2.01 |
| 15 |
Thayse, Eden A (9)
|
NISDSC |
51.24 |
51.38 |
0.14 |
| 16 |
Garigen, Natalie K (7)
|
NIWEBS |
52.10 |
51.40 |
-0.70 |
| 17 |
Galley, Morgan C (8)
|
NIRRST |
1:00.89 |
55.44 |
-5.45 |
| 18 |
Gonzalez, Alexis W (8)
|
NIPACK |
1:07.16 |
58.50 |
-8.66 |
| 19 |
Considine, Elizabeth A (8)
|
NIWEBS |
56.87 |
59.53 |
2.66 |
| 20 |
Bub, Emily R (9)
|
NIGCOM |
57.99 |
59.77 |
1.78 |
| 21 |
Farrell, Kamryn L (6)
|
NIGRSC |
|
1:12.57 |
- |
|
Daggubati, Khyati (9)
|
NIGCOM |
1:06.31 |
NS |
- |