| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Geary, Maria E (8)
|
NIMERC |
47.28 |
45.08 |
-2.20 |
| 2 |
Smith, Chloe A (9)
|
NIGCOM |
49.41 |
47.12 |
-2.29 |
| 3 |
Larzelere, Natalie E (9)
|
NIFAST |
48.98 |
49.89 |
0.91 |
| 4 |
Sanders, Nina P (9)
|
NIFAST |
58.94 |
55.79 |
-3.15 |
| 5 |
Gilbert, Natalie J (8)
|
NIUN |
1:02.59 |
56.37 |
-6.22 |
| 6 |
Chen, Chloe Y (9)
|
NISDSC |
|
57.50 |
- |
| 7 |
Crowley, Lila M (8)
|
NIGRSC |
|
59.12 |
- |
| 8 |
Thayse, Eden A (9)
|
NISDSC |
1:01.67 |
1:03.76 |
2.09 |
| 9 |
Mantell, Norah A (7)
|
NIIRON |
|
1:05.87 |
- |
| 10 |
Chen, Sophie Y (7)
|
NISDSC |
|
1:08.17 |
- |
| 11 |
Galley, Alyssa E (9)
|
NIFAST |
|
1:09.23 |
- |
| 12 |
Larzelere, Charlotte G (7)
|
NIFAST |
|
1:09.81 |
- |
| 13 |
Cady, Sabrina R (9)
|
NIWEBS |
|
1:18.94 |
- |
|
Wagner, Ryleigh J (6)
|
NISDSC |
|
DQ |
- |
|
Considine, Elizabeth A (8)
|
NIWEBS |
|
DQ |
- |
|
Bedford, Marin B (9)
|
NIWEBS |
55.54 |
NS |
- |