| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horner, Aubrey M (12)
|
ORUN |
2:27.94 |
2:26.79 |
-1.15 |
| 2 |
Judish, Saryah A (12)
|
ORWSC |
2:33.47 |
2:33.71 |
0.24 |
| 3 |
Alamein, Sydney N (12)
|
ORBDST |
2:36.62 |
2:34.54 |
-2.08 |
| 4 |
LU, Jinjin (11)
|
ORTEAM |
2:47.23 |
2:38.81 |
-8.42 |
| 5 |
Lamerdin, Louisa (11)
|
ORNST |
2:46.55 |
2:44.08 |
-2.47 |
| 6 |
Hurty, Claire S (11)
|
ORNST |
2:53.13 |
2:49.82 |
-3.31 |
| 7 |
Huling, Penelope M (12)
|
ORTEAM |
3:00.97 |
2:57.58 |
-3.39 |
| 8 |
Buchwald, Amaya A (11)
|
ORTEAM |
|
3:01.77 |
- |
| 9 |
De Lee, McKenna M (11)
|
ORTEAM |
3:09.11 |
3:13.54 |
4.43 |
| 10 |
Larsen, McKenzie D (11)
|
ORTEAM |
3:16.41 |
3:13.89 |
-2.52 |
| 11 |
Snawder, Ruby T (11)
|
ORTEAM |
|
3:14.16 |
- |
| 12 |
Conrady, Katherine E (11)
|
ORNST |
3:42.70 |
3:41.31 |
-1.39 |
|
Brott, Zoey A (11)
|
ORTEAM |
3:13.67 |
DQ |
- |
|
Voelker-Morris, Elise M (11)
|
ORWSC |
|
NS |
- |