| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rahmouni, Noel Joy (7)
|
PVNCAP |
|
45.55 |
- |
| 2 |
Bishop, Alexandra (Lexie) M (7)
|
PVASA |
48.97 |
46.26 |
-2.71 |
| 3 |
Vanlandingham, Avery K (7)
|
PVFBST |
|
49.70 |
- |
| 4 |
Eisenhart, Abigail H (7)
|
PVNCAP |
|
50.02 |
- |
| 5 |
Martin, Amelia B (6)
|
PVASA |
|
52.79 |
- |
| 6 |
Bellerjeau, Marlowe (7)
|
PVASA |
|
54.33 |
- |
| 7 |
Buchanan, Brooke Taylor (7)
|
PVNCAP |
|
54.61 |
- |
| 8 |
Yiengst, Audrey V (6)
|
PVFBST |
1:22.69 |
55.08 |
-27.61 |
| 9 |
Thompson, Addison M (7)
|
PVNCAP |
|
56.17 |
- |
| 10 |
Rasmussen, Anna M (7)
|
PVHACC |
|
59.45 |
- |
| 11 |
Sarathy, Anoushka (7)
|
PVMACH |
|
59.48 |
- |
| 12 |
Foshay, Bella C (7)
|
PVAAC |
|
59.89 |
- |
| 13 |
Beckett, Isla K (7)
|
PVFBST |
|
1:00.07 |
- |
| 14 |
Groves, Charlotte (5)
|
PVASA |
1:10.71 |
1:00.36 |
-10.35 |
| 15 |
Sleighter, Lindsey D (7)
|
PVCSC |
|
1:01.67 |
- |
| 16 |
Shive, Keira X (5)
|
PVNCAP |
|
1:16.14 |
- |
| 17 |
Patel, Meera A (6)
|
PVNCAP |
|
1:26.94 |
- |
|
O'Shaughnessy, Anne M (7)
|
PVFISH |
45.48 |
DQ |
- |
|
Muse, Riley-Simone (7)
|
PVERSC |
|
NS |
- |
|
Pezzimenti, Natalia M (6)
|
PVMACH |
1:12.67 |
NS |
- |
|
Clark, Keegan E (7)
|
PVCSC |
|
NS |
- |
|
Lucchese, Alexandra G (6)
|
PVNCAP |
|
NS |
- |