| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Akins, Jana M (9)
|
PVMACH |
35.62 |
35.73 |
0.11 |
| 2 |
Loehr, Jenna E (10)
|
PVMACH |
37.24 |
37.49 |
0.25 |
| 3 |
Toumazatos, Ana (10)
|
PVMACH |
40.78 |
37.58 |
-3.20 |
| 4 |
Fredericks, Sophie C (9)
|
PVMACH |
40.25 |
40.24 |
-0.01 |
| 5 |
Watson, Marina M (10)
|
PVMACH |
43.80 |
40.55 |
-3.25 |
| 6 |
Sayen, Madison G (9)
|
PVMACH |
45.39 |
42.14 |
-3.25 |
| 7 |
Sribar, Alexa R (10)
|
PVMACH |
45.11 |
43.01 |
-2.10 |
| 8 |
Williams, Zoe E (10)
|
PVMACH |
47.10 |
44.64 |
-2.46 |
| 9 |
Bossle, Caitlin M (10)
|
PVMACH |
42.84 |
45.92 |
3.08 |
| 10 |
Hulsey, Katherine C (9)
|
PVMACH |
1:14.12 |
47.19 |
-26.93 |
| 11 |
Crapco-Wang, Caitlyn L (10)
|
PVMACH |
47.66 |
47.30 |
-0.36 |
| 12 |
Philpott, Annabelle C (9)
|
PVMACH |
48.71 |
47.72 |
-0.99 |
| 13 |
Winston, Sarah H (7)
|
PVMACH |
49.06 |
48.11 |
-0.95 |
| 14 |
Baltz, Hannah L (10)
|
PVMACH |
49.02 |
49.74 |
0.72 |
| 15 |
Hinton, Sophie (8)
|
PVMACH |
|
55.85 |
- |
| 16 |
Golan, Maya R (9)
|
PVMACH |
|
1:00.03 |
- |
|
Harrison, Anjolaoluwa M (9)
|
PVMACH |
48.64 |
NS |
- |
|
Bian, Katherine P (10)
|
PVMACH |
|
NS |
- |