| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kritsky, Josephina (8)
|
PVSNOW |
40.31 |
36.62 |
-3.69 |
| 2 |
Sparrenberger, Riley M (8)
|
PVSNOW |
46.33 |
41.40 |
-4.93 |
| 3 |
Buytenhuys, Julia G (8)
|
PVSNOW |
48.36 |
42.07 |
-6.29 |
| 4 |
Edwards, Avery G (8)
|
PVBWST |
47.55 |
42.12 |
-5.43 |
| 5 |
Landry, Samantha E (8)
|
PVBWST |
|
46.32 |
- |
| 6 |
Albanese, Ava V (8)
|
PVNCAP |
47.57 |
47.21 |
-0.36 |
| 7 |
Wall, Coby B (8)
|
PVBWST |
53.19 |
48.35 |
-4.84 |
| 8 |
Cortez Martinez, Ariana K (7)
|
PVNCAP |
|
48.54 |
- |
| 9 |
Keller, Laurean A (8)
|
PVBWST |
|
49.75 |
- |
| 10 |
McCarney, Maya R (7)
|
PVSNOW |
|
52.81 |
- |
| 11 |
Dolan, Emma G (8)
|
VAWST |
1:09.30 |
53.17 |
-16.13 |
| 12 |
Donovan, Juliet N (6)
|
PVFXFX |
|
54.73 |
- |
| 13 |
Rabe, Skylar R (6)
|
PVNCAP |
|
55.27 |
- |
| 14 |
Clark, Ava L (8)
|
PVBWST |
|
55.41 |
- |
| 15 |
Potturi, Sahasra L (8)
|
PVBWST |
|
56.00 |
- |
| 16 |
Sreniawski, Ella C (8)
|
PVBWST |
|
56.82 |
- |
| 17 |
Gunnerson-Casca, Christina M (7)
|
PVBWST |
|
56.89 |
- |
| 18 |
Block, Lily K (7)
|
PVBWST |
|
1:00.56 |
- |
| 19 |
Clark, Emma E (8)
|
PVBWST |
|
1:01.34 |
- |
|
Alic, Hanna (8)
|
PVBWST |
|
DQ |
- |
|
Sweeney, Dylan (8)
|
PVBWST |
51.00 |
NS |
- |