| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harms, Stella J (6)
|
PVNCAP |
23.29 |
21.90 |
-1.39 |
| 1 |
Abrams, Summer R (6)
|
PVNCAP |
23.79 |
22.93 |
-0.86 |
| 2 |
Bendza, Corinne J (6)
|
PVMACH |
24.81 |
24.62 |
-0.19 |
| 3 |
Farmer, Morgan M (6)
|
PVSNOW |
|
25.01 |
- |
| 4 |
Watts, Emily M (6)
|
PVMACH |
25.53 |
25.31 |
-0.22 |
| 5 |
Matthews, Aubree L (6)
|
PVNCAP |
28.19 |
26.93 |
-1.26 |
| 6 |
Busen, McKinley R (6)
|
PVCSC |
|
28.58 |
- |
| 7 |
Steffens, Clara L (6)
|
PVMACH |
|
30.84 |
- |
| 8 |
Mizzo, Giavanna A (6)
|
PVMACH |
29.02 |
30.89 |
1.87 |
| 9 |
Ter, Olivia E (5)
|
PVNCAP |
40.86 |
31.70 |
-9.16 |
| 10 |
Shive, Keira X (6)
|
PVNCAP |
32.27 |
34.20 |
1.93 |
| 11 |
Yazdani, Ella S (6)
|
PVMACH |
42.25 |
36.49 |
-5.76 |
| 12 |
Wong, Arianna K (5)
|
PVSNOW |
41.22 |
37.23 |
-3.99 |
| 13 |
Hennelly, Makayla N (6)
|
PVNCAP |
54.45 |
39.09 |
-15.36 |
|
Pezzimenti, Natalia M (6)
|
PVMACH |
32.33 |
NS |
- |
|
Lucchese, Alexandra G (6)
|
PVNCAP |
34.64 |
NS |
- |