| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Cheyenne F (13)
|
PVRMSC |
25.09 |
25.14 |
0.05 |
| 2 |
Cornwell, Jamie G (13)
|
PVOCCS |
26.40 |
25.56 |
-0.84 |
| 3 |
Zoltick, Stephanie R (13)
|
PVRMSC |
25.90 |
25.87 |
-0.03 |
| 4 |
Bowrin, Nia A (13)
|
PVRMSC |
25.76 |
26.08 |
0.32 |
| 5 |
Haugen, Emily A (13)
|
PVOCCS |
25.93 |
26.13 |
0.20 |
| 6 |
Pilch, Grace (13)
|
MDMAC |
26.50 |
26.33 |
-0.17 |
| 7 |
Kulikov, Claudia M (13)
|
MDUN |
26.74 |
26.56 |
-0.18 |
| 8 |
Hannam, Emma I (13)
|
PVOCCS |
26.69 |
26.83 |
0.14 |
| 9 |
Burch, Abigail G (13)
|
PVOCCS |
26.46 |
26.92 |
0.46 |
| 10 |
Langan, Riley E (13)
|
PVRMSC |
26.69 |
26.94 |
0.25 |
| 11 |
Hargraves, Jessica R (13)
|
MDMAC |
26.88 |
27.28 |
0.40 |
| 12 |
Frazier, Ryan P (13)
|
MDMAC |
28.26 |
28.71 |
0.45 |
| 13 |
Fitzgerald, Keely M (13)
|
MADST |
28.81 |
30.78 |
1.97 |