| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lesser, Brandon R (10)
|
PVNCAP |
38.80 |
38.18 |
-0.62 |
| 2 |
Weber, William D (10)
|
PVAAC |
37.81 |
38.33 |
0.52 |
| 3 |
Montano, Jesse A (10)
|
PVNCAP |
39.33 |
39.33 |
0.00 |
| 4 |
Makin, Matthew J (10)
|
PVNCAP |
42.02 |
39.94 |
-2.08 |
| 5 |
Chethan, Neel (10)
|
PVLIFE |
43.25 |
43.33 |
0.08 |
| 6 |
Gehley, Michael R (10)
|
PVLIFE |
44.64 |
44.10 |
-0.54 |
| 7 |
Kang, Ian T (9)
|
PVMAC |
45.74 |
45.04 |
-0.70 |
| 8 |
Khoriaty, Liam C (10)
|
PVCSC |
|
46.23 |
- |
| 9 |
Zochowski, Nicholas C (9)
|
PVAAC |
46.92 |
46.48 |
-0.44 |
| 10 |
Rose, Matthew M (10)
|
PVCSC |
44.89 |
47.81 |
2.92 |
| 11 |
Cho, Hanchae J (9)
|
PVMAC |
47.28 |
47.99 |
0.71 |
| 12 |
Downey, Jack E (9)
|
PVNCAP |
51.78 |
49.08 |
-2.70 |
| 13 |
Finnegan, Collin A (9)
|
PVNCAP |
|
49.22 |
- |
| 14 |
Sprinkel, George A (10)
|
PVAAC |
50.10 |
49.33 |
-0.77 |
| 15 |
Alzona, John (10)
|
PVNCAP |
55.75 |
49.77 |
-5.98 |
| 16 |
Kusic, Hayden C (9)
|
PVAAC |
|
49.95 |
- |
| 17 |
Peters, Micah H (10)
|
PVNCAP |
51.65 |
50.08 |
-1.57 |
| 18 |
Elder, Aelan C (9)
|
PVFBST |
|
50.65 |
- |
| 19 |
Sharma, Om V (10)
|
PVNCAP |
51.04 |
52.26 |
1.22 |
| 20 |
Ustundag, Sarp (9)
|
PVNCAP |
|
54.63 |
- |
| 21 |
Sun, Matthew J (10)
|
PVMAC |
|
54.97 |
- |
| 22 |
Canzoneri Crozier, Eli R (9)
|
PVNCAP |
52.36 |
57.97 |
5.61 |
| 23 |
Bak, Noah J (9)
|
PVMAC |
57.03 |
58.24 |
1.21 |
| 24 |
Thompson, Elijah J (9)
|
PVERSC |
59.64 |
1:00.32 |
0.68 |
| 25 |
Bhargava, Nikhil D (10)
|
PVAAC |
55.84 |
1:01.60 |
5.76 |
| 26 |
Peterson, Liam P (10)
|
PVFBST |
|
1:08.30 |
- |
| 27 |
Lenz, Kai A (9)
|
PVAAC |
|
1:09.19 |
- |
| 28 |
Goetcheus, Jax A (9)
|
PVNCAP |
1:08.88 |
1:10.33 |
1.45 |
| 29 |
Dorsett, Micah D (9)
|
PVERSC |
|
1:10.34 |
- |
| 30 |
Hart, David B (9)
|
PVNCAP |
|
1:13.28 |
- |
| 31 |
Poulin, Benjamin R (9)
|
PVAAC |
|
1:13.68 |
- |
| 32 |
Tompkins, Jordan C (10)
|
PVERSC |
|
1:15.41 |
- |
|
Downey, Wyatt W (9)
|
PVNCAP |
|
DQ |
- |