| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harms, Sofia M (8)
|
PVNCAP |
20.26 |
21.01 |
0.75 |
| 2 |
Hooff, Olivia (8)
|
PVYASD |
22.25 |
21.76 |
-0.49 |
| 3 |
Faye, Jaelyn Y (8)
|
PVNCAP |
22.65 |
21.85 |
-0.80 |
| 4 |
Shive, Leah W (8)
|
PVNCAP |
22.34 |
22.37 |
0.03 |
| 5 |
Dourgarian, Charlotte O (8)
|
PVNCAP |
23.10 |
22.70 |
-0.40 |
| 6 |
Mongelli, Megan A (8)
|
PVNCAP |
25.11 |
22.73 |
-2.38 |
| 7 |
Carswell, McKenna B (8)
|
PVNCAP |
25.25 |
22.90 |
-2.35 |
| 8 |
Nguyen, Alyssa T (8)
|
PVNCAP |
25.70 |
24.03 |
-1.67 |
| 9 |
Yuan, Grace S (8)
|
PVFISH |
29.17 |
25.88 |
-3.29 |
| 10 |
Cohen, Lillian C (8)
|
PVFISH |
27.61 |
25.89 |
-1.72 |
| 11 |
Berik, Charlotte X (8)
|
PVFISH |
28.83 |
28.33 |
-0.50 |
| 12 |
Xu, Allison Y (8)
|
PVFISH |
28.57 |
28.54 |
-0.03 |
| 13 |
Chen, Katherine S (8)
|
PVFISH |
|
29.32 |
- |
|
Burakiewicz, Imogen C (8)
|
PVLCL |
24.40 |
NS |
- |
|
Bailey, Violet M (8)
|
PVNCAP |
25.87 |
NS |
- |