| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hilton, Cole W (12)
|
PVNCAP |
27.78 |
26.68 |
-1.10 |
| 2 |
Graham, Nate J (11)
|
PVNCAP |
30.44 |
28.42 |
-2.02 |
| 3 |
Yuan, John (12)
|
PVNCAP |
29.50 |
28.58 |
-0.92 |
| 4 |
Kim, Brandon M (12)
|
PVNCAP |
31.31 |
29.03 |
-2.28 |
| 5 |
Mayhew, Caleb J (12)
|
PVNCAP |
30.78 |
29.15 |
-1.63 |
| 6 |
Osband, Benjamin R (12)
|
PVNCAP |
30.23 |
29.35 |
-0.88 |
| 7 |
Shanfield, Charles E (11)
|
PVNCAP |
31.18 |
29.71 |
-1.47 |
| 8 |
Rudolph, Ian J (12)
|
PVNCAP |
33.30 |
30.86 |
-2.44 |
| 9 |
Abouraya, Mounir M (12)
|
PVUN |
36.48 |
31.12 |
-5.36 |
| 10 |
Meisel, Zachary B (11)
|
PVUN |
31.48 |
31.54 |
0.06 |
| 11 |
Bright, Dmitriy L (11)
|
PVNCAP |
32.71 |
31.63 |
-1.08 |
| 12 |
Floren Smith, Erik O (12)
|
PVNCAP |
|
31.80 |
- |
| 13 |
Idler, Mateo E (12)
|
PVPAC |
33.21 |
31.97 |
-1.24 |
| 14 |
Turner, Riley C (12)
|
PVNCAP |
|
32.40 |
- |
| 15 |
Reynolds, Tory E (12)
|
PVPGKS |
|
32.43 |
- |
| 16 |
Moses II, Jaquada R (12)
|
PVPGPR |
33.53 |
32.47 |
-1.06 |
| 17 |
Hao, August (12)
|
PVPAC |
34.54 |
33.12 |
-1.42 |
| 18 |
Lawry, Hunter L (12)
|
PVPAC |
35.64 |
33.54 |
-2.10 |
| 19 |
McMahon, William N (11)
|
PVPAC |
33.67 |
34.10 |
0.43 |
| 20 |
Adeyemo, Adeoluwa (11)
|
PVPAC |
|
42.55 |
- |
| 21 |
Nguyen, Quoc-Huy N (11)
|
PVPAC |
44.40 |
44.42 |
0.02 |
| 22 |
Johnson-Wright, Jared K (11)
|
PVPGPR |
|
46.21 |
- |
| 23 |
Wampler, Brady A (11)
|
PVPAC |
51.32 |
47.80 |
-3.52 |
| 24 |
Bentsil, Caleb K (12)
|
PVPAC |
59.80 |
1:02.87 |
3.07 |
|
Zamaray, Daniel J (11)
|
PVNCAP |
35.31 |
NS |
- |
|
Zhu, Kerry W (11)
|
PVUN |
36.39 |
NS |
- |