| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Luo, Angelina K (5)
|
PVRMSC |
28.82 |
26.81 |
-2.01 |
| 2 |
Yang, Chloe M (6)
|
PVRMSC |
34.13 |
31.90 |
-2.23 |
| 3 |
Avalos-Restrepo, Natalie (6)
|
PVRMSC |
33.14 |
34.07 |
0.93 |
| 4 |
Heitzmann, Lily S (6)
|
PVRMSC |
33.81 |
34.27 |
0.46 |
| 5 |
Zhou, Elly B (6)
|
PVRMSC |
35.91 |
34.67 |
-1.24 |
| 6 |
Bartholomew, Harper L (5)
|
PVRMSC |
35.25 |
34.90 |
-0.35 |
| 7 |
Kwon, Olivia (6)
|
PVRMSC |
37.47 |
37.09 |
-0.38 |
| 8 |
Lee, Zoey (6)
|
PVRMSC |
|
37.58 |
- |
| 9 |
Wofford, Elise J (5)
|
PVRMSC |
|
40.97 |
- |
| 10 |
Hong, Claire (6)
|
PVRMSC |
|
41.16 |
- |
| 11 |
Groves, Charlotte (5)
|
PVASA |
49.75 |
1:03.51 |
13.76 |
|
Jarczynski, Ashley N (6)
|
PVRMSC |
37.83 |
DQ |
- |
|
Lu, Zoe J (6)
|
PVRMSC |
47.34 |
DQ |
- |
|
Kunkle, Caroline J (6)
|
PVRMSC |
|
DQ |
- |
|
Gu, Mia (6)
|
PVRMSC |
|
DQ |
- |
|
Martin, Amelia B (6)
|
PVASA |
|
DQ |
- |
|
Harms, Stella J (5)
|
PVRMSC |
|
NS |
- |
|
Chen, Zoie (6)
|
PVRMSC |
37.38 |
NS |
- |