| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holmes, Tara M (7)
|
PVAAC |
|
42.27 |
- |
| 2 |
Paradise, Melissa S (7)
|
PVNCAP |
48.27 |
45.12 |
-3.15 |
| 3 |
Bartos, Margaret S (6)
|
PVSDS |
47.15 |
47.11 |
-0.04 |
| 4 |
Blanchard, Brooke C (7)
|
PVNCAP |
|
47.74 |
- |
| 5 |
Lundy, Olivia (7)
|
PVNCAP |
|
49.03 |
- |
| 6 |
Schaeffer, Penelope R (7)
|
PVSDS |
|
50.78 |
- |
| 7 |
Craun, Soraya B (7)
|
PVSDS |
59.02 |
52.16 |
-6.86 |
| 8 |
Snyder, Eleanor L (7)
|
PVSDS |
|
53.02 |
- |
| 9 |
Latcovich, Madeline G (7)
|
PVSDS |
|
53.23 |
- |
| 10 |
Petretich, Maren M (6)
|
PVSDS |
|
57.42 |
- |
| 11 |
Bland, Alique A (7)
|
PVHACC |
56.15 |
57.53 |
1.38 |
| 12 |
Randsmith, Saxon K (6)
|
PVSDS |
|
58.03 |
- |
| 13 |
LoCascio, Vivian A (7)
|
PVSDS |
|
1:15.70 |
- |
| 14 |
Randsmith, Ayka K (6)
|
PVSDS |
|
1:17.99 |
- |
|
Morton, Amelia M (7)
|
PVSDS |
46.83 |
NS |
- |