| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bianchini, Leah C (12)
|
PVSNOW |
37.02 |
34.44 |
-2.58 |
| 2 |
Sian, Isabella R (12)
|
PVBWST |
35.93 |
35.16 |
-0.77 |
| 3 |
Kinloch, Marley L (12)
|
PVSNOW |
39.15 |
38.76 |
-0.39 |
| 4 |
Ramberg, Molly R (11)
|
PVSNOW |
54.14 |
41.24 |
-12.90 |
| 5 |
Greenwood, Lauren (10)
|
PVBWST |
43.08 |
43.55 |
0.47 |
| 6 |
Wilbur, Lilla E (8)
|
PVNCAP |
43.80 |
43.70 |
-0.10 |
| 7 |
Hayward, Audrey I (8)
|
PVBWST |
43.53 |
44.00 |
0.47 |
| 8 |
Seanor, Julianne M (9)
|
PVNCAP |
47.14 |
46.13 |
-1.01 |
| 9 |
Mahadevan, Sukhi V (9)
|
PVSNOW |
50.47 |
48.09 |
-2.38 |
| 10 |
Haas, Allison P (9)
|
PVBWST |
57.07 |
49.48 |
-7.59 |
| 11 |
McCurry, Eva M (9)
|
PVSNOW |
50.50 |
49.87 |
-0.63 |
| 12 |
Chen, Sara L (9)
|
PVSNOW |
55.32 |
52.84 |
-2.48 |
| 13 |
Toporowicz, Alexandra (10)
|
PVSNOW |
55.69 |
55.09 |
-0.60 |
| 14 |
Sparrenberger, Riley M (7)
|
PVSNOW |
1:03.99 |
1:01.16 |
-2.83 |
| 15 |
Livingston, Everly (6)
|
PVBWST |
1:16.19 |
1:02.85 |
-13.34 |
| 16 |
Edwards, Avery G (7)
|
PVBWST |
|
1:03.50 |
- |