| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhang, Ava (7)
|
PVFISH |
54.68 |
47.46 |
-7.22 |
| 2 |
Winston, Sarah H (7)
|
PVMACH |
49.06 |
52.04 |
2.98 |
| 3 |
McDonald, Katherine C (7)
|
PVCSC |
50.06 |
52.83 |
2.77 |
| 4 |
Ru, Alice (7)
|
PVFISH |
1:05.69 |
55.34 |
-10.35 |
| 5 |
Walters, Emily J (7)
|
PVFISH |
54.77 |
55.70 |
0.93 |
| 6 |
Christofferson, Marie N (7)
|
PVMACH |
52.26 |
57.66 |
5.40 |
| 7 |
Curry, Haley A (7)
|
PVMACH |
|
1:01.59 |
- |
| 7 |
Juster, Anne J (7)
|
PVMACH |
|
1:01.59 |
- |
| 9 |
Benisch, Ela R (7)
|
PVYORK |
|
1:04.98 |
- |
| 10 |
Jackson, Madison C (7)
|
PVMACH |
|
1:07.02 |
- |
| 11 |
Casstevens, Lindsey E (7)
|
PVYORK |
|
1:12.10 |
- |
| 12 |
Mann-Webb, Lily M (7)
|
PVMACH |
|
1:16.32 |
- |
|
Nowicki, Ava J (7)
|
PVCSC |
1:01.25 |
DQ |
- |
|
Nguyen, Alyssa T (7)
|
PVNCAP |
|
DQ |
- |
|
McMullin, Fay L (7)
|
PVPM |
|
DQ |
- |