| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chanin, Isabella M (10)
|
PVRMSC |
53.65 |
39.51 |
-14.14 |
| 2 |
Grijalba, Isabella (10)
|
PVRMSC |
|
39.88 |
- |
| 3 |
Young, Aida H (10)
|
PVCSC |
40.62 |
39.96 |
-0.66 |
| 4 |
Hastings, Clara W (9)
|
PVNCAP |
|
40.16 |
- |
| 5 |
Kwon, Alyssa L (10)
|
PVRMSC |
|
40.56 |
- |
| 6 |
Santino, Victoria M (10)
|
PVLIFE |
44.17 |
41.46 |
-2.71 |
| 7 |
Majeed-Hall, Aliyah M (9)
|
PVCSC |
57.15 |
43.18 |
-13.97 |
| 8 |
Heitzmann, Madison G (10)
|
PVRMSC |
|
43.41 |
- |
| 9 |
Schwieters, Julie K (10)
|
PVRMSC |
48.04 |
43.66 |
-4.38 |
| 10 |
Ross, Elinor A (10)
|
PVASA |
49.50 |
43.77 |
-5.73 |
| 11 |
Gartzke, Monika T (9)
|
PVCSC |
|
44.84 |
- |
| 12 |
Finn, Elizabeth C (10)
|
PVASA |
44.22 |
44.92 |
0.70 |
| 13 |
Liu, Isabel X (10)
|
PVFBST |
55.67 |
45.12 |
-10.55 |
| 14 |
Quispe, Katherine G (10)
|
PVRMSC |
46.82 |
45.23 |
-1.59 |
| 15 |
Chang, Charlotte (9)
|
PVRMSC |
50.34 |
46.76 |
-3.58 |
| 16 |
Bodner, Leila G (10)
|
PVCSC |
48.94 |
47.43 |
-1.51 |
| 17 |
Bellerjeau, Schuyler A (10)
|
PVASA |
|
48.63 |
- |
| 18 |
Ipakchi, Ariana (9)
|
PVASA |
53.63 |
48.66 |
-4.97 |
| 19 |
Chen, Elizabeth L (8)
|
PVRMSC |
46.98 |
49.04 |
2.06 |
| 20 |
Levine, Lauren F (10)
|
PVASA |
|
49.51 |
- |
| 21 |
Bortnick, Naomi M (10)
|
PVASA |
51.35 |
50.46 |
-0.89 |
| 22 |
Baffer, Bennett R (9)
|
PVNCAP |
|
51.27 |
- |
| 23 |
Goldstein, Naomi S (10)
|
PVASA |
51.57 |
52.05 |
0.48 |
| 24 |
Huynh, Amy N (10)
|
PVNCAP |
52.35 |
52.52 |
0.17 |
| 25 |
Hinds, Virginia C (9)
|
PVASA |
56.78 |
53.75 |
-3.03 |
| 26 |
Muragaki, Reina (9)
|
PVRMSC |
54.40 |
1:00.41 |
6.01 |
| 27 |
Mirestean, Alina R (10)
|
PVASA |
1:00.16 |
1:02.34 |
2.18 |
| 28 |
Wang, Felcia C (9)
|
PVRMSC |
1:00.09 |
1:06.71 |
6.62 |