| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Watts, Courtney M (11)
|
PVFISH |
5:35.13 |
5:25.47 |
-9.66 |
| 2 |
Blake, Katherine M (12)
|
PVRMSC |
5:30.77 |
5:30.52 |
-0.25 |
| 3 |
Van Dyke, Olivia S (12)
|
MDBAAC |
5:38.56 |
5:36.67 |
-1.89 |
| 4 |
Tyer, Gabrielle N (12)
|
MDRAC |
5:40.34 |
5:39.26 |
-1.08 |
| 5 |
Malone, Margaret B (12)
|
MDRAC |
5:39.66 |
5:42.45 |
2.79 |
| 6 |
Johnson, Kiera S (11)
|
PVFISH |
5:50.85 |
5:44.00 |
-6.85 |
| 7 |
Novak, Victoria N (12)
|
MDBAAC |
5:42.28 |
5:45.44 |
3.16 |
| 8 |
Arciaga, Savannah J (12)
|
MDGBSA |
5:54.67 |
5:50.92 |
-3.75 |
| 9 |
Li, Mia (12)
|
MDEST |
5:52.32 |
5:53.34 |
1.02 |
| 10 |
Iimi, Rin (12)
|
PVRMSC |
5:50.93 |
5:53.37 |
2.44 |
| 11 |
Hendrickson, Margaret R (12)
|
MDEST |
6:01.68 |
5:55.82 |
-5.86 |
| 12 |
Ellsworth, Ashley P (10)
|
MDBAAC |
6:03.50 |
5:57.11 |
-6.39 |
| 13 |
Betancourt, Gabriela I (12)
|
PVRMSC |
5:52.92 |
5:57.18 |
4.26 |
| 14 |
Gurri, Lucia M (12)
|
MDGBSA |
6:00.77 |
5:59.58 |
-1.19 |
| 15 |
Li, Grace (12)
|
PVRMSC |
6:15.90 |
6:02.54 |
-13.36 |
| 16 |
Traub, Charlotte S (12)
|
PVRMSC |
6:04.03 |
6:11.43 |
7.40 |
| 17 |
Miller, Kelsie (11)
|
PVRMSC |
6:21.22 |
6:13.65 |
-7.57 |
| 18 |
Zhang, Clara Ann (12)
|
PVRMSC |
6:28.80 |
6:27.85 |
-0.95 |