| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Towers, Zachary D (20)
|
NISTAR |
23.69 |
24.48 |
0.79 |
| 2 |
Maurer, Michael O (17)
|
NISTAR |
24.96 |
24.89 |
-0.07 |
| 3 |
Harper, Benjamin C (16)
|
NITTSC |
25.10 |
25.23 |
0.13 |
| 4 |
Acosta, Isaiah J (17)
|
NISTAR |
25.56 |
25.39 |
-0.17 |
| 5 |
Staples, Graeme A (15)
|
NISTAR |
28.12 |
27.38 |
-0.74 |
| 6 |
Matteson, Carter J (16)
|
NISTAR |
28.74 |
27.49 |
-1.25 |
| 7 |
Burkett, Robert O (15)
|
NISTAR |
31.14 |
28.23 |
-2.91 |
| 8 |
Garcia, Brandon J (15)
|
NITTSC |
30.17 |
28.56 |
-1.61 |
| 9 |
Strittmatter, Blaise A (15)
|
NISTAR |
30.40 |
28.95 |
-1.45 |
| 10 |
Eisenmann, Zachary M (15)
|
NISTAR |
30.98 |
31.50 |
0.52 |