| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harper, Benjamin C (16)
|
NITTSC |
22.25 |
22.79 |
0.54 |
| 2 |
Maurer, Michael O (17)
|
NISTAR |
22.70 |
22.87 |
0.17 |
| 3 |
Duszynski, Andrew M (23)
|
NISTAR |
22.03 |
22.99 |
0.96 |
| 4 |
Towers, Zachary D (20)
|
NISTAR |
22.38 |
23.07 |
0.69 |
| 5 |
Acosta, Isaiah J (17)
|
NISTAR |
23.59 |
23.74 |
0.15 |
| 6 |
Walker, Matthew D (16)
|
NITTSC |
23.84 |
24.50 |
0.66 |
| 7 |
Staples, Graeme A (15)
|
NISTAR |
25.16 |
25.33 |
0.17 |
| 8 |
Matteson, Carter J (16)
|
NISTAR |
25.64 |
25.47 |
-0.17 |
| 9 |
Burkett, Robert O (15)
|
NISTAR |
27.15 |
26.39 |
-0.76 |
| 10 |
Christie, Alex N (18)
|
NIEAST |
25.97 |
26.93 |
0.96 |
| 11 |
Strittmatter, Blaise A (15)
|
NISTAR |
26.22 |
27.17 |
0.95 |
| 12 |
Eisenmann, Zachary M (15)
|
NISTAR |
28.87 |
28.40 |
-0.47 |