| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carey, Juliana E (16)
|
GAGA |
2:23.60 |
2:25.05 |
1.45 |
| 2 |
Ludwick, Makayla C (16)
|
SESES |
2:25.11 |
2:25.10 |
-0.01 |
| 3 |
Wright, Mollie J (17)
|
WTWT |
2:24.42 |
2:25.63 |
1.21 |
| 4 |
Berger, Julie C (16)
|
SESES |
2:29.91 |
2:26.13 |
-3.78 |
| 5 |
Osburn, Aspyn n (16)
|
WTWT |
2:28.79 |
2:26.58 |
-2.21 |
| 6 |
Turner, Haley M (15)
|
NCNCS |
2:25.31 |
2:27.20 |
1.89 |
| 7 |
Payne, Grayson E (15)
|
SESES |
2:23.55 |
2:27.38 |
3.83 |
| 8 |
Gasper, Ashton K (16)
|
NCNCS |
2:28.55 |
2:27.43 |
-1.12 |
| 9 |
Herren, Parker S (17)
|
KYKY |
2:29.68 |
2:27.80 |
-1.88 |
| 10 |
Quincy, Lauren E (17)
|
STSTX |
2:26.12 |
2:28.67 |
2.55 |
| 11 |
Stewart, Mary K (17)
|
SESES |
2:26.75 |
2:28.96 |
2.21 |
| 12 |
Ozyurekoglu, Elif (15)
|
KYKY |
2:30.38 |
2:29.23 |
-1.15 |
| 13 |
Parker, Dailey M (18)
|
WTWT |
2:33.53 |
2:29.73 |
-3.80 |
| 14 |
Mahaffey, Hannah K (18)
|
GAGA |
2:25.93 |
2:29.77 |
3.84 |
| 15 |
Graf, Julianna R (16)
|
FLFL |
2:31.89 |
2:30.08 |
-1.81 |
| 16 |
Riggs, Abigail R (17)
|
WVWV |
2:29.78 |
2:30.15 |
0.37 |
| 17 |
Auerbach, Gabrielle V (15)
|
GAGA |
2:32.61 |
2:30.74 |
-1.87 |
| 18 |
Abruzzino, Adriana B (16)
|
WVWV |
2:37.42 |
2:33.23 |
-4.19 |
| 19 |
Brantley, Lawson C (15)
|
NCNCS |
2:28.05 |
2:34.48 |
6.43 |
| 20 |
Friesz, Rae L (17)
|
STSTX |
2:29.20 |
2:34.55 |
5.35 |
| 21 |
Witcher, Avery R (17)
|
KYKY |
2:31.46 |
2:34.84 |
3.38 |
| 22 |
Johnson, Sarah M (17)
|
SCSCZT |
2:32.95 |
2:35.03 |
2.08 |
| 23 |
Ellison, Hannalee K (15)
|
NCNCS |
2:34.44 |
2:37.01 |
2.57 |
| 24 |
Pierce, Brittany C (18)
|
LALA |
2:32.02 |
2:37.04 |
5.02 |
| 25 |
Vega, Emma N (15)
|
NTNTX |
2:34.80 |
2:39.87 |
5.07 |
| 26 |
Mistric, Meghan T (18)
|
LALA |
2:44.28 |
2:42.97 |
-1.31 |
| 27 |
Zinobile, Allison C (15)
|
KYKY |
2:51.53 |
2:48.36 |
-3.17 |
| 28 |
Cox, Jessica L (15)
|
STSTX |
3:01.14 |
2:49.61 |
-11.53 |