| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Livingston, Olivia M (15)
|
AMJCCS |
58.60 |
58.51 |
-0.09 |
| 2 |
Feng, Hannah A (17)
|
STUN-0 |
58.41 |
58.93 |
0.52 |
| 3 |
Grimme, Chloe D (15)
|
FLPS |
58.63 |
58.96 |
0.33 |
| 4 |
Cordovi, Emily (16)
|
FGGRSC |
59.67 |
58.99 |
-0.68 |
| 5 |
Skirboll, Zoe M (12)
|
AMRXA |
59.05 |
59.10 |
0.05 |
| 6 |
Hinton, Jacey L (14)
|
NEBGSC |
58.96 |
59.21 |
0.25 |
| 7 |
Higgs, Lillian L (16)
|
NCMAC |
59.79 |
59.25 |
-0.54 |
| 8 |
Evans, Kelsey C (18)
|
GUCFSC |
59.63 |
59.47 |
-0.16 |
| 9 |
Cash, Joanie (14)
|
MRLIAC |
59.00 |
59.52 |
0.52 |
| 10 |
Vannote, Elizabeth A (15)
|
NCMAC |
1:02.98 |
59.98 |
-3.00 |
| 11 |
Buckley, Mary K (17)
|
NEKING |
1:01.01 |
1:00.38 |
-0.63 |
| 12 |
Burzynski, Monika (16)
|
NJFSPY |
59.98 |
1:00.72 |
0.74 |
| 13 |
Derikoletis, Keiko L (16)
|
CACITI |
1:00.78 |
1:01.58 |
0.80 |
| 14 |
Graham, Emily M (17)
|
ILWEST |
59.50 |
1:02.16 |
2.66 |