| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Earley, Cristin (14)
|
CTCT |
2:28.12 |
2:26.90 |
-1.22 |
| 2 |
Nierhoff, Luisa (13)
|
MRMETR |
2:27.86 |
2:29.19 |
1.33 |
| 3 |
Stanford, Catherine M (14)
|
MRMETR |
2:28.75 |
2:29.61 |
0.86 |
| 4 |
Tetreault, Madison G (14)
|
NENE |
2:29.06 |
2:29.80 |
0.74 |
| 5 |
Waggoner, Alexandra M (14)
|
MAMA |
2:34.46 |
2:31.23 |
-3.23 |
| 6 |
Sokolowski, Suzanne N (13)
|
NINI |
2:29.76 |
2:31.36 |
1.60 |
| 7 |
Amuan, Malia T (13)
|
NENE |
2:25.31 |
2:31.50 |
6.19 |
| 8 |
Brown, Eliza K (13)
|
NENE |
2:33.60 |
2:32.12 |
-1.48 |
| 9 |
Whelan, Anna P (14)
|
VAVSI |
2:32.65 |
2:32.36 |
-0.29 |
| 10 |
Kopal, Alexandra (14)
|
VAVSI |
2:32.60 |
2:33.04 |
0.44 |
| 11 |
Blake, Katherine M (13)
|
PVPV |
2:33.81 |
2:33.19 |
-0.62 |
| 12 |
O'Neill, Claire R (14)
|
MAMA |
2:34.20 |
2:33.37 |
-0.83 |
| 13 |
Larkin, Ava R (13)
|
NENE |
2:34.08 |
2:33.40 |
-0.68 |
| 14 |
Huang, Isabel S (13)
|
AMAM |
2:32.11 |
2:33.44 |
1.33 |
| 15 |
Craig, Kendall A (14)
|
AMAM |
2:31.76 |
2:33.46 |
1.70 |
| 16 |
Attar, Clara M (13)
|
PVPV |
2:31.11 |
2:34.09 |
2.98 |
| 17 |
Mueller, Elexis G (13)
|
VAVSI |
2:33.82 |
2:34.60 |
0.78 |
| 18 |
Welker, Sydney E (14)
|
MAMA |
2:34.04 |
2:35.00 |
0.96 |
| 19 |
Wright, Brynn C (13)
|
NENE |
2:34.13 |
2:35.39 |
1.26 |
| 20 |
Shepard, Margaret T (14)
|
VAVSI |
2:32.73 |
2:35.44 |
2.71 |
| 21 |
Hiller, Isabelle R (14)
|
NENE |
2:34.23 |
2:35.77 |
1.54 |
| 22 |
Davis, Korina E (13)
|
VAVSI |
2:34.32 |
2:38.09 |
3.77 |
| 23 |
Lew, Katie G (14)
|
MRMETR |
2:34.24 |
2:38.89 |
4.65 |
| 24 |
Polecki, Julia (14)
|
NJNJS |
2:33.03 |
2:40.74 |
7.71 |
| 25 |
Nebor, Brooke (13)
|
CTCT |
2:34.83 |
2:41.39 |
6.56 |
| 26 |
Leung, Adelaide Y (14)
|
ADADLC |
2:42.09 |
2:41.85 |
-0.24 |
| 27 |
Burke, Emma S (13)
|
ADADLC |
2:48.09 |
2:48.59 |
0.50 |
|
Hawkins, Emma L (14)
|
NINI |
2:32.09 |
DQ |
- |
|
Parchman, Morwenna J (14)
|
NJNJS |
2:40.56 |
NS |
- |
|
Morisi, Kiani B (14)
|
MRMETR |
2:30.17 |
NS |
- |