| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Craig, Molly C (17)
|
NIHHTA |
25.43 |
25.41 |
-0.02 |
| 2 |
St Rose-Finear, Sydney R (16)
|
NIVICT |
25.19 |
26.36 |
1.17 |
| 3 |
Craig, Catherine K (15)
|
NIHHTA |
26.77 |
26.79 |
0.02 |
| 4 |
Ritz, Erin (15)
|
NIVICT |
26.80 |
26.87 |
0.07 |
| 5 |
Turek, Sabrina R (16)
|
NIVICT |
25.71 |
26.90 |
1.19 |
| 6 |
Lettera, Paige A (16)
|
NIVICT |
26.02 |
27.33 |
1.31 |
| 7 |
Sargent, Savannah R (15)
|
NIVICT |
26.31 |
27.35 |
1.04 |
| 8 |
Gartner, Sarah N (15)
|
NIGCOM |
26.24 |
27.55 |
1.31 |
| 9 |
Farman, Morgan K (16)
|
NIVICT |
26.45 |
27.65 |
1.20 |
| 10 |
Winseman, Isabelle A (15)
|
NIVICT |
27.12 |
28.06 |
0.94 |
| 11 |
Ludlow, Allison G (17)
|
NIMERC |
25.83 |
28.21 |
2.38 |
| 12 |
Keister, Elise M (16)
|
NIHHTA |
27.15 |
29.41 |
2.26 |
| 13 |
Roperti, Marissa A (15)
|
NISEAS |
30.07 |
31.10 |
1.03 |