| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scamacca, Michael A (10)
|
NIHAST |
42.76 |
41.84 |
-0.92 |
| 2 |
Kabunov, Aleksei V (8)
|
NIUN-J |
44.83 |
42.31 |
-2.52 |
| 3 |
Milliman, Kaden M (10)
|
NIWGSC |
42.34 |
43.46 |
1.12 |
| 4 |
Maynard, Calvin J (10)
|
NIWGSC |
52.31 |
49.36 |
-2.95 |
| 5 |
Mesh, Dante M (10)
|
NIHAST |
48.65 |
49.62 |
0.97 |
| 6 |
Hedden, Dominick J (10)
|
NIVICT |
|
57.27 |
- |
| 7 |
Healy, Owen D (10)
|
NIVICT |
56.66 |
58.60 |
1.94 |
| 8 |
Kelley, Gage T (9)
|
NISDSC |
1:00.49 |
58.96 |
-1.53 |
| 9 |
Eaton, Alexander L (8)
|
NISDSC |
59.30 |
1:06.81 |
7.51 |
| 10 |
Edgecombe, Chase M (10)
|
NIHAST |
53.88 |
1:08.16 |
14.28 |
|
Ellickson, Cameron B (10)
|
NISDSC |
|
DQ |
- |
|
Bajus, Nathan J (9)
|
NIVICT |
|
DQ |
- |
|
Locke, Benjamin D (10)
|
NIVICT |
54.45 |
NS |
- |
|
Ray, Gavin M (9)
|
NIVICT |
1:11.22 |
NS |
- |
|
Stahl, Reed P (9)
|
NIVICT |
52.05 |
NS |
- |