| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Silva, Veronica Y (13)
|
VANOVA |
3:02.02 |
3:04.06 |
2.04 |
| 2 |
Kluger, Pearl L (13)
|
VAWAC |
3:09.18 |
3:10.36 |
1.18 |
| 3 |
Kiger, Ally S (14)
|
VAWAC |
3:11.69 |
3:11.85 |
0.16 |
| 4 |
Fuller, Abigail B (13)
|
VAACAC |
3:08.33 |
3:11.86 |
3.53 |
| 5 |
Masterson, Caroline A (13)
|
VATSU |
3:18.45 |
3:14.55 |
-3.90 |
| 6 |
Cram, Laura E (14)
|
VANOVA |
3:27.92 |
3:21.58 |
-6.34 |
| 7 |
DeGuzman, Zoe B (14)
|
VAACAC |
3:25.33 |
3:22.74 |
-2.59 |
| 8 |
Smith, Bryana L (13)
|
VAWAC |
3:23.68 |
3:23.47 |
-0.21 |
| 9 |
DeRosier, Olivia S (13)
|
VANOVA |
3:24.64 |
3:24.37 |
-0.27 |
| 10 |
Young, Kelly E (14)
|
VAACAC |
3:39.33 |
3:32.82 |
-6.51 |
| 11 |
McMahan, Isabelle E (13)
|
VAWAC |
3:39.94 |
3:44.31 |
4.37 |
| 12 |
Crampton, Isabella E (13)
|
VATSU |
3:43.45 |
3:45.68 |
2.23 |