| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Neal, Madison F (12)
|
NCTYDE |
33.94 |
34.76 |
0.82 |
| 2 |
Wiles, Morgan E (11)
|
NCTYDE |
34.82 |
35.25 |
0.43 |
| 3 |
Frondoza, Audrianne R (11)
|
NCTYDE |
36.86 |
35.52 |
-1.34 |
| 4 |
Mellor, Tatum N (12)
|
NCTYDE |
38.58 |
35.54 |
-3.04 |
| 5 |
Moehrke, Taylor M (11)
|
NCTYDE |
41.09 |
36.45 |
-4.64 |
| 6 |
Lewis, Lauren E (11)
|
NCTYDE |
36.44 |
36.57 |
0.13 |
| 7 |
Holshouser, Maci (12)
|
NCRACY |
39.41 |
37.99 |
-1.42 |
| 8 |
Shlien-Dellinger, Phoenix S (12)
|
NCWNCY |
52.42 |
38.98 |
-13.44 |
| 9 |
Parks, Ella F (12)
|
NCTYDE |
41.47 |
39.08 |
-2.39 |
| 10 |
O'Shaughnessey, Ella C (9)
|
SCYCSC |
46.64 |
46.21 |
-0.43 |
| 11 |
Bonavilla, Jacqueline G (7)
|
SCYCSC |
43.07 |
53.15 |
10.08 |
|
Keefauver, Eva G (11)
|
NCWNCY |
46.42 |
NS |
- |