| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Neal, Madison F (12)
|
NCTYDE |
32.46 |
31.91 |
-0.55 |
| 2 |
Parks, Ella F (12)
|
NCTYDE |
36.27 |
32.59 |
-3.68 |
| 3 |
Holshouser, Maci (12)
|
NCRACY |
33.77 |
33.09 |
-0.68 |
| 4 |
Wiles, Morgan E (11)
|
NCTYDE |
33.54 |
33.45 |
-0.09 |
| 5 |
Moehrke, Taylor M (11)
|
NCTYDE |
34.57 |
33.67 |
-0.90 |
| 6 |
Mellor, Tatum N (12)
|
NCTYDE |
34.23 |
33.68 |
-0.55 |
| 7 |
Frondoza, Audrianne R (11)
|
NCTYDE |
35.00 |
34.41 |
-0.59 |
| 8 |
Lewis, Lauren E (11)
|
NCTYDE |
35.60 |
34.98 |
-0.62 |
| 9 |
Shlien-Dellinger, Phoenix S (12)
|
NCWNCY |
41.62 |
35.95 |
-5.67 |
| 10 |
O'Shaughnessey, Ella C (9)
|
SCYCSC |
41.17 |
36.82 |
-4.35 |
| 11 |
Bonavilla, Jacqueline G (7)
|
SCYCSC |
45.63 |
45.26 |
-0.37 |