| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Plogger, Alexis N (16)
|
VALY |
2:23.98 |
2:27.48 |
3.50 |
| 2 |
Salgado, Lauren I (17)
|
VAODAC |
2:23.81 |
2:28.07 |
4.26 |
| 3 |
Olsen, Grace A (16)
|
VAWAC |
2:34.59 |
2:30.64 |
-3.95 |
| 4 |
Erquiaga, Carrington M (17)
|
VALY |
2:32.04 |
2:33.38 |
1.34 |
| 5 |
Rossin, Hannah M (16)
|
VASTAT |
2:37.66 |
2:34.78 |
-2.88 |
| 6 |
Kirkman, Madison E (16)
|
VAODAC |
2:36.60 |
2:36.78 |
0.18 |
| 7 |
Brenner, Blythe T (15)
|
VAODAC |
2:50.07 |
2:42.63 |
-7.44 |
| 8 |
Pendleton, Makayla D (18)
|
VALY |
2:36.73 |
2:43.31 |
6.58 |
| 9 |
Geiszler, Peyton K (16)
|
VANOVA |
2:42.03 |
2:45.47 |
3.44 |
| 10 |
Gabriele, Grace E (15)
|
VAGATR |
2:45.97 |
2:45.83 |
-0.14 |
| 11 |
Parker, Sophia L (15)
|
NCSTAR |
2:47.70 |
2:48.82 |
1.12 |
| 12 |
Hall, Sydney V (18)
|
VAODAC |
2:50.54 |
2:49.03 |
-1.51 |
| 13 |
Kiracofe, Mycala (16)
|
VAGATR |
3:01.14 |
2:57.72 |
-3.42 |
| 14 |
Bennett, Emily G (15)
|
VARAPP |
|
3:07.59 |
- |
| 15 |
Martin, Danae N (16)
|
VAODAC |
3:05.06 |
3:11.99 |
6.93 |
|
Kreutter, Katelynn A (16)
|
VAODAC |
|
NS |
- |
|
Hester, Virginia G (15)
|
VANOVA |
2:35.87 |
NS |
- |