| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kim, Dohyun (14)
|
ORLOSC |
24.40 |
24.73 |
0.33 |
| 2 |
Peng, Steven G (13)
|
ORLOSC |
25.08 |
24.79 |
-0.29 |
| 3 |
Davies, Lauren K (13)
|
ORCS |
24.95 |
25.63 |
0.68 |
| 4 |
Thistlethwaite, William I (12)
|
ORLOSC |
26.48 |
26.36 |
-0.12 |
| 5 |
McMorrow, Kaitlyn M (12)
|
ORCURR |
26.83 |
26.81 |
-0.02 |
| 6 |
Williams, Elli (13)
|
ORMST |
26.26 |
27.10 |
0.84 |
| 7 |
Kraljev, Mila N (13)
|
ORTDPS |
26.60 |
27.17 |
0.57 |
| 8 |
Waldner, Caitlin (13)
|
ORMVA |
26.85 |
27.32 |
0.47 |
| 9 |
Heaton, Kendra C (12)
|
ORMVA |
28.70 |
27.98 |
-0.72 |
| 10 |
Graves, Michelle T (11)
|
ORHRST |
28.82 |
28.46 |
-0.36 |
| 11 |
Schneider, Cassidy M (11)
|
ORLOSC |
28.95 |
28.47 |
-0.48 |
| 12 |
Scherwinski, Hannah B (13)
|
ORLHST |
29.98 |
30.45 |
0.47 |
| 13 |
Webster, Henry P (12)
|
ORLHST |
30.00 |
31.87 |
1.87 |
|
Tyler, Chloe R (13)
|
ORSHSC |
27.00 |
NS |
- |