| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rados, Lucy M (10)
|
NITWST |
40.24 |
38.68 |
-1.56 |
| 2 |
Fabiano, Mackenzie G (10)
|
NIHHSC |
43.71 |
41.29 |
-2.42 |
| 3 |
Price, Elizabeth E (10)
|
NIFKVL |
44.22 |
45.18 |
0.96 |
| 4 |
Canestrari, Willow G (10)
|
NITWST |
42.52 |
46.35 |
3.83 |
| 5 |
Loehfelm, Haley E (9)
|
NITWST |
42.74 |
47.11 |
4.37 |
| 6 |
Girard, Sydney V (9)
|
NIEAST |
54.26 |
48.63 |
-5.63 |
| 7 |
Wagner, Ava I (10)
|
NITWST |
51.12 |
52.95 |
1.83 |
| 8 |
Brackenridge, Peyton Q (9)
|
NITWST |
54.32 |
56.26 |
1.94 |
| 9 |
Schmitt, Kimberly R (9)
|
NITTSC |
54.21 |
57.07 |
2.86 |
| 10 |
Reader, Caelyn E (9)
|
NITWST |
1:01.48 |
1:02.96 |
1.48 |
|
Kellogg, Elsa E (9)
|
NITWST |
|
DQ |
- |
|
Seelig, Jordan L (9)
|
NIEAST |
54.30 |
NS |
- |