| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walier, Nora E (6)
|
NITWST |
59.52 |
1:00.24 |
0.72 |
| 2 |
Spino, Kadence E (8)
|
NITWST |
57.89 |
1:00.86 |
2.97 |
| 3 |
Loehfelm, Madelyn G (8)
|
NITWST |
1:01.76 |
1:02.43 |
0.67 |
| 4 |
Carducci, Elizabeth G (7)
|
NITWST |
|
1:03.64 |
- |
| 5 |
Goldstein, Madison R (8)
|
NITWST |
|
1:03.82 |
- |
| 6 |
Kirchberger, Addison M (8)
|
NIHAMB |
|
1:06.75 |
- |
| 7 |
Cook, Hannah R (7)
|
NIHAMB |
1:18.87 |
1:14.51 |
-4.36 |
| 8 |
Bell, Lila R (8)
|
NITWST |
|
1:15.11 |
- |
| 9 |
Jankowski, LilaEve L (7)
|
NIGWST |
|
1:16.27 |
- |
|
Garland, Paige S (7)
|
NIHAMB |
|
DQ |
- |
|
Scott, Macey M (7)
|
NIHAMB |
|
DQ |
- |
|
Bruzgul, Vaughn S (8)
|
NITWST |
|
DQ |
- |
|
Wilson, Juliet (8)
|
NITWST |
|
DQ |
- |