| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huntress, Maya V (10)
|
NIBAAC |
|
34.03 |
- |
| 2 |
Reilly, Bridget F (10)
|
NISTAR |
35.29 |
34.91 |
-0.38 |
| 3 |
Puzanovova, Veronika A (10)
|
NIBAAC |
42.22 |
38.05 |
-4.17 |
| 4 |
Golden, Lauren B (10)
|
NIBAAC |
40.78 |
38.11 |
-2.67 |
| 5 |
Rasmusson, Henrietta E (10)
|
NICLSC |
44.03 |
39.93 |
-4.10 |
| 6 |
Burnham, Madeline (10)
|
NIVELO |
40.15 |
40.08 |
-0.07 |
| 7 |
Oneill, Meghan R (10)
|
NICLSC |
|
40.38 |
- |
| 8 |
King, Izzy M (10)
|
NIEAST |
40.96 |
44.23 |
3.27 |
| 9 |
Pisa, Cadence E (10)
|
NIBAAC |
|
45.04 |
- |
| 10 |
Lawrence, Kaleigh M (10)
|
NIFAST |
39.17 |
45.27 |
6.10 |
| 11 |
DeFedericis, Sheeanne (10)
|
NICLSC |
46.08 |
46.84 |
0.76 |
| 12 |
Zugelder, Shannon (10)
|
NIVELO |
|
49.55 |
- |
| 13 |
Wands, Lucille M (10)
|
NIVELO |
52.93 |
50.37 |
-2.56 |
| 14 |
Washington, Ashanti M (10)
|
NISTAR |
1:06.61 |
53.41 |
-13.20 |
| 15 |
Seelig, Jordan L (10)
|
NIEAST |
49.62 |
57.14 |
7.52 |
|
Yost, Sydney (10)
|
NIVELO |
|
DQ |
- |
|
Alessi, Luciana M (10)
|
NIBAAC |
57.68 |
NS |
- |