| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Anderson, Madden (11)
|
NIVELO |
36.42 |
35.04 |
-1.38 |
| 2 |
Morey, Celia R (11)
|
NIBAAC |
43.83 |
38.16 |
-5.67 |
| 3 |
Philbrick, Mylie E (11)
|
NIVELO |
40.17 |
38.66 |
-1.51 |
| 4 |
Casler, Hannah R (11)
|
NIFAST |
43.31 |
38.80 |
-4.51 |
| 5 |
Weisenburg, Brylee M (11)
|
NISTAR |
42.43 |
38.95 |
-3.48 |
| 6 |
Strittmatter, Amelia C (11)
|
NISTAR |
40.44 |
39.14 |
-1.30 |
| 7 |
Arroyo, Lucia R (11)
|
NICLSC |
|
39.30 |
- |
| 8 |
Gachette, Marisa B (11)
|
NIVELO |
41.27 |
40.08 |
-1.19 |
| 9 |
King, Skylar L (11)
|
NIEAST |
35.20 |
41.22 |
6.02 |
| 10 |
Wamsley, Sarah E (11)
|
NIVELO |
40.39 |
41.90 |
1.51 |
| 11 |
Barillari, Emma (11)
|
NIVELO |
39.24 |
43.06 |
3.82 |
| 12 |
Peiper, Reagan S (11)
|
NIBAAC |
42.78 |
43.16 |
0.38 |
| 13 |
Reukauf, Anna R (11)
|
NISTAR |
49.41 |
43.81 |
-5.60 |
| 14 |
Umansky, Isabella C (11)
|
NIBAAC |
46.71 |
45.87 |
-0.84 |
| 15 |
Urbanek, Addison E (11)
|
NICLSC |
53.16 |
46.44 |
-6.72 |
| 16 |
Kalbfus, Taylor R (11)
|
NIFAST |
53.43 |
46.90 |
-6.53 |
| 17 |
Haines, Kaylee N (11)
|
NISTAR |
|
47.86 |
- |
| 18 |
Przybysz, Tara (11)
|
NIBAAC |
52.56 |
48.63 |
-3.93 |
| 19 |
Arcadi, Kayla A (11)
|
NIVELO |
51.37 |
48.72 |
-2.65 |
| 20 |
Balcom, Lucy W (11)
|
NIBAAC |
44.80 |
48.79 |
3.99 |
| 21 |
Burg, Julia S (11)
|
NIBAAC |
|
49.15 |
- |
| 22 |
Gawron, Samantha G (11)
|
NICLSC |
54.86 |
49.73 |
-5.13 |
| 23 |
Glorioso, Morgan M (11)
|
NIFAST |
56.71 |
50.36 |
-6.35 |
| 24 |
Ferrari, Abigail C (11)
|
NIFAST |
53.03 |
52.97 |
-0.06 |
| 25 |
Galley, Alyssa E (11)
|
NIFAST |
55.33 |
56.60 |
1.27 |
| 26 |
McCully, Hannah M (11)
|
NISHAQ |
|
59.16 |
- |
| 27 |
Kappel, Alexandra H (11)
|
NIVELO |
|
1:07.76 |
- |
|
Schwartzbeck, Elisabeth M (11)
|
NIFAST |
1:13.95 |
DQ |
- |
|
Tothero, Madeleine S (11)
|
NISTAR |
48.91 |
NS |
- |
|
Whitley-Grassi, Ava E (11)
|
NISTAR |
|
NS |
- |
|
Kasubowski, Cassie A (11)
|
NIEAST |
37.03 |
NS |
- |