| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Garrett, Audrey V (10)
|
WIMAC |
6:09.36 |
5:55.09 |
-14.27 |
| 2 |
Corro, Bridget A (10)
|
WIWEST |
6:11.61 |
6:01.40 |
-10.21 |
| 3 |
Finstad, Madeline A (10)
|
WIGBSC |
6:25.79 |
6:03.21 |
-22.58 |
| 4 |
Inkmann, Lily J (10)
|
WIWBSC |
6:25.89 |
6:03.37 |
-22.52 |
| 5 |
Worgull, Audrey C (10)
|
WILAKE |
6:13.65 |
6:04.86 |
-8.79 |
| 6 |
Sina, Olivia M (10)
|
WIOCSC |
5:37.82 |
6:08.19 |
30.37 |
| 7 |
Meyer, Emerson J (9)
|
WISWAT |
6:27.22 |
6:08.49 |
-18.73 |
| 8 |
Johnson, Haley A (10)
|
WIWEST |
6:29.84 |
6:11.20 |
-18.64 |
| 9 |
McQueen, Scout V (9)
|
WIWYNS |
6:18.50 |
6:12.02 |
-6.48 |
| 10 |
Gruber, Zoe A (9)
|
WIHSSB |
6:32.93 |
6:16.56 |
-16.37 |
| 11 |
Block, Alyse (9)
|
WIOCSC |
6:23.23 |
6:17.45 |
-5.78 |
| 12 |
Goodbear, Elena Q (10)
|
WISWAT |
6:41.45 |
6:21.30 |
-20.15 |
| 13 |
Schuch, Erin L (10)
|
WIASC |
5:55.05 |
6:25.90 |
30.85 |
| 14 |
Jondle, Brynn G (10)
|
WIMAC |
6:38.43 |
6:30.22 |
-8.21 |
| 15 |
Miller, Lillian R (10)
|
WINLAC |
6:48.53 |
6:31.92 |
-16.61 |
| 16 |
Gundrum, Addisyn M (10)
|
WIWBSC |
6:45.48 |
6:32.83 |
-12.65 |
| 17 |
Borroughs, Cheyenne R (9)
|
WISTAR |
6:35.13 |
6:33.06 |
-2.07 |
| 18 |
Faris, Kennedy E (10)
|
WIOCSC |
6:41.05 |
6:33.63 |
-7.42 |
| 19 |
Dietschweiler, Kiersten K (9)
|
WIOSHY |
6:53.36 |
6:34.30 |
-19.06 |
| 20 |
Roszak, Molly I (10)
|
WIMKE |
6:52.29 |
6:45.34 |
-6.95 |
| 21 |
Baumbich, Colleen A (10)
|
WILAXY |
6:52.45 |
6:46.20 |
-6.25 |
| 22 |
Johnson, Taylor N (10)
|
WIMFSC |
6:49.76 |
6:54.73 |
4.97 |
| 23 |
Vanden Burgt, Kaitlyn K (10)
|
WIWAVE |
7:10.97 |
7:05.81 |
-5.16 |