| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shao, Emma X (8)
|
ADGUY |
41.39 |
40.56 |
-0.83 |
| 2 |
Stoup, Madelyn L (8)
|
ADCPP |
46.90 |
43.10 |
-3.80 |
| 3 |
Dheeradhada, Arya J (8)
|
ADALBS |
45.17 |
43.28 |
-1.89 |
| 4 |
Zhang, Annabelle Y (8)
|
ADCPP |
40.05 |
43.35 |
3.30 |
| 5 |
Walter, Emma L (8)
|
ADCPP |
53.25 |
45.07 |
-8.18 |
| 6 |
Rogowski, Isabella S (8)
|
ADSCHE |
43.81 |
45.65 |
1.84 |
| 7 |
Adams, Michelle E (7)
|
ADCPP |
45.85 |
46.06 |
0.21 |
| 8 |
Braun, Julia L (8)
|
ADNTS |
46.64 |
46.21 |
-0.43 |
| 9 |
Stewart Fraser, Thornton R (7)
|
ADALBS |
45.35 |
46.34 |
0.99 |
| 10 |
Zen, Dominique A (8)
|
ADDELM |
47.16 |
48.54 |
1.38 |
| 11 |
Allinson, Mae L (8)
|
ADDELM |
51.90 |
53.52 |
1.62 |
| 12 |
Relyea, Cadence G (7)
|
ADDELM |
55.78 |
57.94 |
2.16 |
| 13 |
Krenav, Anna E (7)
|
ADEELS |
1:00.97 |
58.33 |
-2.64 |
| 14 |
White, Saramarie E (8)
|
ADALBS |
1:06.34 |
1:06.04 |
-0.30 |
| 15 |
Matte, Bailey P (6)
|
ADGLFY |
1:18.66 |
1:07.49 |
-11.17 |
| 16 |
O'Donnell, Bridgett M (8)
|
ADEELS |
1:06.42 |
1:10.53 |
4.11 |
| 17 |
Banik, Noel Y (7)
|
ADALBS |
1:14.22 |
1:15.57 |
1.35 |
|
Shukla, Aarya O (8)
|
ADSCHE |
54.17 |
NS |
- |