| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sosa, Lylah E (8)
|
GUSHAC |
50.03 |
46.71 |
-3.32 |
| 2 |
Laurito, Carmen S (8)
|
GUAQUA |
49.44 |
49.53 |
0.09 |
| 3 |
Carandang, Chloe A (8)
|
GUSWAT |
48.91 |
50.55 |
1.64 |
| 4 |
Schauss, Ingrid A (8)
|
GUAQUA |
53.97 |
53.02 |
-0.95 |
| 5 |
Dickamore, Kathrin I (8)
|
GUSHRK |
56.47 |
56.56 |
0.09 |
| 6 |
Montgomery, Emerson E (8)
|
GULJAC |
57.68 |
1:00.64 |
2.96 |
| 7 |
Mutch, Allye C (8)
|
GUAQUA |
|
1:04.13 |
- |
| 8 |
McAuliffe, Caitlin C (8)
|
GUSHRK |
1:07.10 |
1:06.81 |
-0.29 |
| 9 |
Avila, Jessica E (8)
|
GUHCAP |
1:30.86 |
1:07.24 |
-23.62 |
| 10 |
Herbst, Emily F (8)
|
GUUN-S |
1:15.24 |
1:08.10 |
-7.14 |
| 11 |
Torres, Ruby G (8)
|
GUHCAP |
1:18.80 |
1:09.38 |
-9.42 |
|
Payne, Avery S (8)
|
GUAQUA |
1:02.56 |
DQ |
- |
|
Estrada, Mairyn (8)
|
GUHCAP |
|
DQ |
- |
|
Jacobson, Katherine R (8)
|
GUSHRK |
|
DQ |
- |
|
Baig, Sophia (8)
|
GUAQUA |
|
NS |
- |
|
Gray, Stormie (8)
|
GUSHRK |
|
NS |
- |