| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burdick, Riley E (12)
|
ADHAWK |
41.82 |
41.52 |
-0.30 |
| 2 |
Tafilowski, Julianna M (11)
|
ADDELM |
42.73 |
41.91 |
-0.82 |
| 3 |
Wieczorek, Jazmyn A (12)
|
ADDELM |
41.89 |
43.61 |
1.72 |
| 4 |
Fitzgerald, Catherine G (11)
|
ADDELM |
45.64 |
44.35 |
-1.29 |
| 5 |
Carsillo, Gianna I (11)
|
ADDELM |
44.14 |
44.93 |
0.79 |
| 6 |
Ohl, Madeline A (12)
|
ADBFIN |
49.08 |
45.70 |
-3.38 |
| 7 |
Murphy, Alice M (12)
|
ADDELM |
54.01 |
47.69 |
-6.32 |
| 8 |
Greaux, Lauren A (11)
|
ADDELM |
43.59 |
48.47 |
4.88 |
| 9 |
Sheshany, Dalya H (12)
|
ADHAWK |
47.17 |
48.64 |
1.47 |
| 10 |
Szymanski, Hannah E (11)
|
ADGRGF |
53.26 |
49.47 |
-3.79 |
| 11 |
Linehan, Kathryn J (11)
|
ADGRGF |
50.44 |
49.55 |
-0.89 |
| 12 |
Cunningham, Isabelle N (11)
|
ADDELM |
53.10 |
54.91 |
1.81 |
|
Thompson, Cora A (11)
|
ADUSAM |
44.66 |
NS |
- |