| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wendling, Anya E (11)
|
ADDELM |
46.77 |
32.70 |
-14.07 |
| 2 |
McDermott, Maeve E (12)
|
ADUSAM |
33.22 |
33.21 |
-0.01 |
| 3 |
Domitrovits, Charlee E (12)
|
ADHAWK |
35.75 |
35.48 |
-0.27 |
| 4 |
Ohl, Madeline A (12)
|
ADBFIN |
40.52 |
37.71 |
-2.81 |
| 5 |
Fraley, Kaiya R (11)
|
ADBFIN |
38.26 |
37.86 |
-0.40 |
| 6 |
Wieczorek, Jazmyn A (12)
|
ADDELM |
36.86 |
38.56 |
1.70 |
| 7 |
Kennelly, Nora E (11)
|
ADGRGF |
42.35 |
41.34 |
-1.01 |
| 8 |
Carsillo, Gianna I (11)
|
ADDELM |
41.48 |
42.47 |
0.99 |
| 9 |
Eisler, Alexis A (11)
|
ADBSPA |
|
46.29 |
- |
| 10 |
Brandl, Lauren C (12)
|
ADBSPA |
|
48.43 |
- |
| 11 |
Cunningham, Isabelle N (11)
|
ADDELM |
47.36 |
54.06 |
6.70 |
|
Mitchell, Madeline M (12)
|
ADDELM |
44.41 |
NS |
- |