| # | Swimmer | Team | Seed | Time | Diff |
| 3 |
Shpritz, Annalyse O (10)
|
MDEST |
47.65 |
46.33 |
-1.32 |
| 7 |
Sun, Sofie R (10)
|
MDEST |
|
49.61 |
- |
| 9 |
Pickering, Folasade A (10)
|
MDCAA |
|
51.53 |
- |
| 10 |
Dengler, Emily C (10)
|
MDFSC |
55.61 |
51.65 |
-3.96 |
| 11 |
Hamilton, Kathryn D (10)
|
MDCAA |
47.51 |
51.87 |
4.36 |
| 12 |
Ivall, Olivia P (10)
|
MDFAST |
55.51 |
52.63 |
-2.88 |
| 13 |
Winters, Emma R (10)
|
MDFSC |
52.00 |
53.57 |
1.57 |
| 14 |
Brashear, Johanna H (10)
|
MDFAST |
56.38 |
53.96 |
-2.42 |
| 15 |
Kochunov, Anita P (10)
|
MDCAA |
54.38 |
56.24 |
1.86 |
| 17 |
Curry, McKinley A (10)
|
MDBCSC |
1:10.85 |
57.41 |
-13.44 |
| 18 |
Demory, Elizbabeth N (10)
|
MDFAST |
1:03.72 |
59.76 |
-3.96 |
| 19 |
Johnson, Maleah A (10)
|
MDBCSC |
1:03.08 |
1:05.19 |
2.11 |
|
Hittle, Ava E (10)
|
MDEST |
|
DQ |
- |
|
Siglar, Lucy A (10)
|
MDHSST |
|
NS |
- |