| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hardee, Christin A (16)
|
GUCFSC |
5:13.04 |
5:23.67 |
10.63 |
| 2 |
Schlimper, Clara F (15)
|
GUCFSC |
5:18.02 |
5:24.05 |
6.03 |
| 3 |
Hanson, Morgan C (15)
|
GUCFSC |
5:46.09 |
5:25.92 |
-20.17 |
| 4 |
Duester, Allyson B (16)
|
GUCFSC |
5:24.83 |
5:28.62 |
3.79 |
| 5 |
Ervin, Anna E (16)
|
GUCFSC |
5:37.97 |
5:30.71 |
-7.26 |
| 6 |
Zimmerman, Kathryn D (16)
|
GUUN-C |
5:11.13 |
5:30.87 |
19.74 |
| 7 |
Argueta, Nabila A (15)
|
GUCFSC |
5:26.35 |
5:36.76 |
10.41 |
| 8 |
Praczko, Tamara (17)
|
GUCFSC |
5:30.45 |
5:37.71 |
7.26 |
| 9 |
Schnibben, Mikayla K (17)
|
GUCFSC |
5:14.58 |
5:40.78 |
26.20 |
| 10 |
Killinger, Kaitlyn (15)
|
GUKATY |
5:38.38 |
5:45.03 |
6.65 |
| 11 |
Trujillo, Leyla M (15)
|
GUKATY |
5:39.27 |
5:59.72 |
20.45 |
| 12 |
Walter, Gabriela A (17)
|
GUKATY |
5:46.98 |
6:06.90 |
19.92 |
| 13 |
Bowers, Abby J (15)
|
GUTASC |
5:38.40 |
6:14.03 |
35.63 |
| 14 |
Hassmann, Tori A (15)
|
GUKATY |
|
6:16.01 |
- |